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Real-time generation of user defined event markers and event journaling.

Trace math analysis functions such as antilog, average, combine, XY combine, differentiate, integrate, linear fit, and peak finder, FRET Calculator, 1-4 Exponential fitting, Global Analysis.

Square, single area of interest photometry in real-time.

The Macro editor is simply one of the easiest to use ever designed by HORIBA! This allows the researcher to increment the start excitation wavelength, emission wavelength, number of points per second, increase temperature and a host of other acquisition modes. You simply select the function you want and then apply it to the loop. This means you do not have to be a programmer or understand a scripting language to implement user defined macros.

Modular Design - If the out of the box acquisition protocols do not quite fit your applications, the PTI RatioMaster Macro editor allows you to create your own specialized experiments by a drag and drop command library into a loop logic.

Ready to run common experiments “out of the box” with minimal input from the user. In addition, you can create your own templates.

Day to Day Routine - Session templates for common experiments are provided.

This feature allows researchers to get started inexpensively and then add functionality later as their research grows. Components can be added to the system to increase functionality.

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Open Architecture FelixGX™ software also allows for maximum flexibility for your future needs with our modular software architecture.

No proprietary binary formats allow the researcher to manipulate data with peace of mind that their data remains in its original form and can be opened in any spreadsheet program.

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Data Integrity - We know how valuable your data is, which is why we use an XML file to archive and store experimental data.This allows correlation of contractility information with calcium concentration.

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Data is collected and analyzed by proprietary Windows “FelixGX” based advanced fluorescence software.Īn optional Sarcomere Length module is available to measure the changes in sarcomere length and whole cell length as a function of time. All system functions are under computer control. Analog detection is used when emitted light is relatively intense low light levels are detected by photon counting.

Emitted light is collected from the sample and passed through a photometer having a bilateral translatable iris and a viewing eyepiece to a switchable analog/photon counting photomultiplier detector. Alternating excitation wavelengths are selected by a computer-controlled high-speed random access monochromator coupled to a (user specified/supplied) inverted or upright, fluorescence microscope with a liquid light guide. A xenon arc lamp provides high intensity, continuous broadband illumination. The PTI RatioMaster is capable of dynamic ratio fluorescence measurements on a millisecond timescale.

More importantly, PTI RatioMaster is fast enough to provide essential dynamic information about ion concentrations and interactions with other molecules, drugs or added reagents. Unlike conventional intensity based fluorescence microscopy, ratio-fluorescence eliminates the effects of photo-bleaching, cell path length variation, non-uniformity of dye loading, non-uniform field of view, making ratio-fluorescence the most accurate method to determine intracellular ion concentrations. Ratio-fluorescence microscopy is the ultimate tool to study dynamic events in live cells and cellular structures. PTI RatioMaster is a monochromator-based wide-field microscope system for measuring ratio-fluorescence or fluorescence dye intensity of labeled proteins in nanomolar concentrations.

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